Nucleoside hydrolase from Leishmania (L.) donovani is an antigen diagnostic for visceral leishmaniasis.
نویسندگان
چکیده
Débora M. Santana a,b, Gulnara P. Borja-Cabrera b, Edilma Paraguai de Souza b, Nancy R. Sturm a, Clarisa B. Palatnik de Sousa b, David A. Campbell * a Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of California, 10833 Le Conte Avenue, Los Angeles, CA 90095-1747, USA b Instituto de Microbiologia*/Professor Paulo de Góes, CCS, Cidade Universitária, Ilha do Fundão, UFRJ, CP 68040, CEP 21944-590 Rio de Janeiro, RJ, Brazil
منابع مشابه
F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
The Leishmania (Leishmania) donovani nucleoside hydrolase NH36 is the main antigen of the Leishmune® vaccine and one of the promising candidates for vaccination against visceral leishmaniasis. The antigenicity of the N-terminal (F1), the central (F2), or the C-terminal recombinant domain (F3) of NH36 was evaluated using peripheral blood mononuclear cells (PBMC) from individuals infected with L....
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Background: The therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of leishmania antigens. In this report, we produced several mAb aga...
متن کاملrKLO8, a Novel Leishmania donovani – Derived Recombinant Immunodominant Protein for Sensitive Detection of Visceral Leishmaniasis in Sudan
BACKGROUND For effective control of visceral leishmaniasis (VL) in East Africa, new rapid diagnostic tests are required to replace current tests with low sensitivity. The aim of this study is to improve diagnosis of VL in East Africa by testing a new antigen from an autochthonous L. donovani strain in Sudan. METHODOLOGY AND PRINCIPLE FINDINGS We cloned, expressed and purified a novel recombin...
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عنوان ژورنال:
- Molecular and biochemical parasitology
دوره 120 2 شماره
صفحات -
تاریخ انتشار 2002